yitW [2019-06-27 13:48:58]

iron-sulphur cluster assembly factor

locus: BSU11160

pI: 4.10

mw: 11.18 kDa

protein length: 102 aa

gene length: 309 bp

function: assembly of iron-sulphur clusters

product: iron-sulphur cluster assembly factor

essential: no

synonyms: sufT

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.3|Biosynthesis of iron-sulfur clusters]

Gene

Coordinates: 1,192,254 → 1,192,562

The protein

Protein family

• UPF0195 family (according to Swiss-Prot)

[SW|Domains]

• DUF59 [pubmed|28589301]

Structure

• [PDB|3LNO] (from B. antracis, 58% identity)

Expression and Regulation

Operons

• genes: [gene|9592D944FE0863A3099E396D9A96F60E61D6C01C|yitV]-[gene|DB374B763EE496FA5F0904686384D94170CE47E3|yitW]
  description: [Pubmed|22383849]

• genes: [gene|DB374B763EE496FA5F0904686384D94170CE47E3|yitW]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A509 (yitW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/509 NBRP B. subtilis, Japan]
• BKE11160 (Δ[gene|DB374B763EE496FA5F0904686384D94170CE47E3|yitW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCCCTCACTCCTTTT, downstream forward: _UP4_TAAAACAAAAAAGGACAGCC
• BKK11160 (Δ[gene|DB374B763EE496FA5F0904686384D94170CE47E3|yitW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTCCCTCACTCCTTTT, downstream forward: _UP4_TAAAACAAAAAAGGACAGCC

References

Reviews

• 28589301

Research papers

• 27517714,27671355